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Image Search Results
Journal: Scandinavian journal of immunology
Article Title: Mucosal-associated invariant T cells modulate innate immune cells and inhibit colon cancer growth.
doi: 10.1111/sji.13391
Figure Lengend Snippet: FIGURE 3 Activation of MAIT cells enhance killing against colon cancer cells. (A) Experimental setup of human whole leukocyte isolation and killing assay co-culture with COLO 205. (B) Percent specific lysis of COLO 205 co-cultured with human whole leukocytes with or without 5-A-RU/MGO stimulation using a 4-h Calcein Release Assay. (C) Frequency of CFSE+ cells after overnight co-culture of human whole leukocytes with COLO 205 with or without 5-A-RU/MGO stimulation for 16 h by flow cytometry. Each dot represents a sample from an individual healthy donor and data were collected from two independent experiments. Figure created using BioRender.com. *p < 0.05 by Wilcoxon ranked test.
Article Snippet: For flow cytometry- based killing assay, 100,000
Techniques: Activation Assay, Isolation, Co-Culture Assay, Lysis, Cell Culture, Release Assay, Flow Cytometry